Fig. 1
From: Dormant non-culturable Mycobacterium tuberculosis retains stable low-abundant mRNA
Samples used for RNA-seq analysis. a Experimental flow. Formation of a ‘zero-CFU’ M. tuberculosis population under K+ deficiency and a moderate concentration of rifampicin (5 μg ml−1). Filled squares, CFU counts; filled circles, MPN counts. The experiment was repeated five times with similar results. A typical experiment is shown. The standard deviation for CFU did not exceed 10 to 20 % for the CFU mean and 20 to 30 % for the MPN mean. b The quantity of total mRNA (M values) in the DE, DM, and DL cultures to that in the Log cultures (after normalization to 16S rRNA)