Fig. 5

Cleavage of 23S rRNA. a Non-denaturing agarose gel electrophoresis of total RNA isolated from the Log, DE, DM, and DL M. tuberculosis cultures. b Northern blotting with the probes complementary to 5′ and 3′ ends of 23S rRNA from the Log and DM cells. c Primer extension analysis with the primer PE_667 complementary to 23S rRNA. RNA was isolated from the Log, DE, DM, and DL cells. The cloned 23S rDNA fragment was also analyzed by Sanger sequencing with PE_667. The arrow indicates the cleavage site. d The structure of helix 24 and the adjacent region of M. tuberculosis 23S rRNA. The cleavage site is indicated by the arrow. The structure was adopted from www.rna.icmb.utexas.edu [78] and modified