Skip to main content
Fig. 7 | BMC Genomics

Fig. 7

From: Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress

Fig. 7

Comparison of floral regulatory and heat shock protein gene expression between the HT and HS lines. a RT-PCR analysis of mRNA expression of flowering-time control genes (Bo-FT, Bo-FLC1, and Bo-AP1) and heat stress-responsive genes (Bo-HSP70 and Bo-HSP20) under different temperatures at 50 DPG. 18S rRNA was used as an internal control. b Floral meristem identity genes (Bo-AP1) after 70 DPG at 15 °C. c Floral meristem identity genes (Bo-AP1 and Bo-CAL) after 100, 120, and 140 DPG at 22 °C. d Microarray heatmap of genes encoding plant heat shock proteins, including HSP70 and HSP20 gene family. The cDNA sequences encoding proteins were annotated on the basis of Arabidopsis genes in TAIR (The Arabidopsis Information Resource). The black box indicates the difference in expression level between HT and HS at 22 °C. The log2 expression values derived from 3 biological replications corresponding to each sample were shown

Back to article page