Fig. 8

Genotypic variation and characterization of the heat-tolerant 'AVS1' (HT1) and heat-sensitive 'BR1 op' (HS) genotypes in broccoli during head formation under heat stress. a Different head-forming capacity of HT1 and HS lines after 128 DPG at 22 °C. Scale bars = 1 cm. b Semi-quantitative RT-PCR analysis of mRNA expression of Bo-FT and Bo-FLC1 after 50 DPG at 22 °C. 18S rRNA was used as an internal control. c Stem-loop qRT-PCR showing relative expression of the selected known and novel miRNAs in comparison with HT1 and HS lines. U6 was used as a loading control. d Comparison of the gene expression profiles of their miRNA putative targets between HT1 and HS lines using qRT-PCR analysis. 18S rRNA was used as an internal control. Asterisks denote significant differences from the HT (Student’s t-test, *P < 0.05, ***P < 0.005)