Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress

Table 2 Differentially expressed known miRNAs in shoot apexes between HT and HS broccoli under heat stress

Family	miRNA	miRNA sequence (5′- > 3′)	HT count^a	HS count^a	Fold change expression (HT/HS)	p-value	Signature (p < 0.001)
miR156	bol-miR156a	UGACAGAAGAGAGUGAGCACA	10.64	4.46	2.37	2.16E-16	TRUE
	bol-miR156b	UUGACAGAAGAUAGAGAGCAC	25.38	9.22	2.73	1.97E-46	TRUE
	bol-miR156e	UGACAGAAGAGAGUGAGCAC	10.05	3.89	2.56	1.10E-17	TRUE
	bol-miR156l	UGACAGAAGAGAGUGAGCAC	9.79	3.96	2.45	4.18E-16	TRUE
	bol-miR156n	UGACAGAAGAGAGUGAGCAC	9.05	4.19	2.14	6.61E-12	TRUE
miR157	bol-miR157a	UUGACAGAAGAUAGAGAGCAC	24.09	9.22	2.59	8.77E-41	TRUE
	bol-miR157b	UUGACAGAAGAUAGAGAGCAC	24.01	9.18	2.59	1.02E-40	TRUE
miR166	bol-miR166d*	GGAUUGUUGUCUGGCACGAGG	0.78	1.83	0.42	6.66E-04	TRUE
miR169	bol-miR169f	UGAGCCAAGGAUGACUUGCCG	11.27	5.45	2.05	2.87E-13	TRUE
	bol-miR169m	UGAGCCAAAGAUGACUUGCCG	40.93	13.10	3.10	1.75E-87	TRUE
miR172	bol-miR172d	AGAAUCUUGAUGAUGCUGCAG	22.35	1.37	16.16	1.33E-129	TRUE
miR391	bol-miR391	UUCGCAGGAGAGAUAGCGCCA	0.07	0.65	0.11	3.22E-04	TRUE
miR395	bol-miR395a	CUGAAGUGUUUGGGGGAACUC	2.29	0.11	19.87	4.30E-15	TRUE
	bol-miR395b	CUGAAGUGUUUGGGGGGACUC	39.53	6.78	5.78	3.61E-148	TRUE
	bol-miR395c	CUGAAGUGUUUGGGGGGACUC	37.86	5.75	6.53	9.13E-153	TRUE
miR398	bol-miR398b	UGUGUUCUCAGGUCACCCCUG	1.07	4.27	0.25	1.40E-13	TRUE
	bol-miR398b*	GGGUCGAUAUGAGAACACAUG	5.54	13.64	0.40	1.36E-22	TRUE
miR400	bol-miR400	UAUGAGAGUAUUAUAAGUCAC	0.59	2.63	0.22	1.27E-09	TRUE
miR408	bol-miR408a*	ACAGGGAACAAGCAGAGCAUG	39.86	14.89	2.65	5.64E-69	TRUE
miR827	bol-miR827*	UUUGUUGAUUGACAUCUAUGC	0.07	2.48	0.03	6.05E-18	TRUE

^acount was normalized as transcripts per million (TPM)
*miRNA* is from the stem–loop precursor miRNAs, which is processed into the miRNA/miRNA* duplex

ISSN: 1471-2164