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Table 6 Summary of RT-PCR results for female-biased DEGs

From: Gonadal transcriptomic analysis and differentially expressed genes in the testis and ovary of the Pacific white shrimp (Litopenaeus vannamei)

Sequence ID Bioinformatic analysis Fold change Semi-quantitative PCR validation RQ (O/T) qPCR validation (T-test) Annotation
Unigene15643_All OB 2.05 / −2.32 ** SUMO-activating enzyme subunit 1
Unigene13333_All OB 40.71 / −1.75 N Hypothetical protein
Unigene13199_All OB 13.27 / −1.72 * Facilitated trehalose transporter Tret1
Unigene4124_All OB 2.2 / −1.62 ** DNA ligase 1
Unigene3159_All OB 8.44 / −1.44 ** --
Unigene5760_All OB 4.17 / −1.32 N Tail muscle elongation factor 1 gamma
Unigene9807_All OB 52.44 / −1.3 * --
CL1556.Contig1_All OB 6.53 / −1.11 N Hemolymph clottable protein
Unigene4414_All OB 3.61 / −1.01 N DNA replication complex GINS protein
Unigene13906_All OB 2.81 / 1.12 N NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
Unigene12464_All OB 3.49 / 1.37 ** Vitelline membrane protein
CL1780.Contig2_All OB 4.55 / 1.76 ** Cytosolic MnSOD
Unigene4283_All OB 6.66 / 1.8 ** Ubiquitin carboxyl-terminal hydrolase isozyme L5
Unigene5769_All OB 5.67 / 2.17 ** Mannose-P-dolichol utilization defect 1 protein homolog
Unigene2752_All OB 3.21 / 2.83 ** 39S ribosomal protein L27, mitochondrial
CL1767.Contig3_All OB 36.82 / 3.76 ** Vitellogenin
Unigene10823_All OB 3.77 / 4.49 ** GJ22360 [Drosophila virilis]
Unigene7488_All OB 10.38 / 6 ** Megaderma lyra mitochondrial aldehyde dehydrogenase 2 (Aldh2) gene
CL745.Contig1_All OB 73.15 / 10.4 ** JHE-like carboxylesterase 1
CL1422.Contig1_All OB 35.44 / 21.85 ** Penaeus (Litopenaeus) vannamei microsatellite TUMXLv8.67 sequence
CL355.Contig1_All OB 41.48 / 23.46 ** Metallothionein
Unigene12371_All OB 67.42 / 30.75 ** Serine protease-like protein
Unigene2772_All OB 129.19 / 52.27 ** Crustacyanin-A2 subunit
Unigene10728_All OB 187.39 / 57.32 ** Putative microtubule-associated protein
CL160.Contig1_All OB 274.18 / 58.75 ** Mucin-19
Unigene9289_All OB 188.85 / 62.16 ** ATP binding cassette transmembrane transporter
CL1757.Contig1_All OB 6.5 / 68.51 ** Pol-like protein
Unigene944_All OB 232.28 / 78.45 ** --
Unigene2582_All OB 82.88 / 83.62 ** Peroxidase
Unigene15565_All OB 561 / 165.67 ** PREDICTED: hypothetical protein
Unigene15673_All OB 230.04 / 180.28 ** Hypothetical protein UY3_06274
CL1544.Contig3_All OB 2.35 / 279.59 ** Toll protein
Unigene7394_All OB 186.3 / 309.91 ** Penaeus monodon progestin membrane receptor component 1 (PGMRC1)
CL92.Contig2_All OB 99.85 / 743.69 ** Penaeus monodon polehole-like protein Mrna
Unigene10229_All OB 64.19 / 754.82 ** --
Unigene11618_All OB 1070.04 / 938.62 ** --
CL92.Contig3_All OB 103.95 / 1017.81 ** Polehole-like protein
Unigene2102_All OB 695.22 / 2497.98 ** Vitellogenin receptor
CL989.Contig2_All OB 992.51 / 3232.49 ** Thrombospondin protein
CL2390.Contig2_All OB 226.25 / 3345.08 ** Cyclin B
Unigene9017_All OB 215.42 / 3401.83 ** Cyclin B
CL2487.Contig2_All OB 1015.41 / 7095.22 ** Thrombospondin
CL1646.Contig1_All OB 1025.74 / 11552.92 ** Thrombospondin-type laminin G domain and EAR repeat-containing protein
Unigene905_All OS / Not specific 6.07 ** Zebrafish DNA sequence from clone CH211-150 K4 in linkage group 1
CL496.Contig2_All OS / Not specific 23.23 ** Glutamate receptor ionotropic, NMDA 3A
Unigene7304_All OS / Not specific 72.23 ** X-linked retinitis pigmentosa GTPase regulator [Tupaia chinensis]
Unigene12972_All OS / Not specific 117.13 ** Peroxidasin
CL1169.Contig2_All OS / Not specific 315.15 ** PREDICTED: uncharacterized protein
Unigene12309_All OS / Specific / / Dual oxidase maturation factor 1
Unigene8249_All OS / Specific / / PREDICTED: guanine nucleotide-binding protein G(q) subunit alpha-like
Unigene21442_All OS / Specific / / --
Unigene9242_All OS / Specific / / PREDICTED: MD-2-related lipid-recognition protein-like
Unigene1049_All OS / Specific / / Amyloid beta A4 protein
CL57.Contig2_All OS / Specific / / Gamma-interferon-inducible lysosomal thiol reductase
CL711.Contig1_All OS / Specific / / Mucin-5 AC (Fragments)
Unigene4288_All OS / Specific / / --
Unigene859_All OS / Specific / / --
  1. OB ovary biased, OS ovary specific, RQ relative expression level
  2. Asterisks indicate significant difference between ovary and testis expression of the gene by QPCR test ( *P<0.05, **P<0.01)