Table 6 Summary of RT-PCR results for female-biased DEGs
Sequence ID | Bioinformatic analysis | Fold change | Semi-quantitative PCR validation | RQ (O/T) | qPCR validation (T-test) | Annotation |
|---|---|---|---|---|---|---|
Unigene15643_All | OB | 2.05 | / | −2.32 | ** | SUMO-activating enzyme subunit 1 |
Unigene13333_All | OB | 40.71 | / | −1.75 | N | Hypothetical protein |
Unigene13199_All | OB | 13.27 | / | −1.72 | * | Facilitated trehalose transporter Tret1 |
Unigene4124_All | OB | 2.2 | / | −1.62 | ** | DNA ligase 1 |
Unigene3159_All | OB | 8.44 | / | −1.44 | ** | -- |
Unigene5760_All | OB | 4.17 | / | −1.32 | N | Tail muscle elongation factor 1 gamma |
Unigene9807_All | OB | 52.44 | / | −1.3 | * | -- |
CL1556.Contig1_All | OB | 6.53 | / | −1.11 | N | Hemolymph clottable protein |
Unigene4414_All | OB | 3.61 | / | −1.01 | N | DNA replication complex GINS protein |
Unigene13906_All | OB | 2.81 | / | 1.12 | N | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 |
Unigene12464_All | OB | 3.49 | / | 1.37 | ** | Vitelline membrane protein |
CL1780.Contig2_All | OB | 4.55 | / | 1.76 | ** | Cytosolic MnSOD |
Unigene4283_All | OB | 6.66 | / | 1.8 | ** | Ubiquitin carboxyl-terminal hydrolase isozyme L5 |
Unigene5769_All | OB | 5.67 | / | 2.17 | ** | Mannose-P-dolichol utilization defect 1 protein homolog |
Unigene2752_All | OB | 3.21 | / | 2.83 | ** | 39S ribosomal protein L27, mitochondrial |
CL1767.Contig3_All | OB | 36.82 | / | 3.76 | ** | Vitellogenin |
Unigene10823_All | OB | 3.77 | / | 4.49 | ** | GJ22360 [Drosophila virilis] |
Unigene7488_All | OB | 10.38 | / | 6 | ** | Megaderma lyra mitochondrial aldehyde dehydrogenase 2 (Aldh2) gene |
CL745.Contig1_All | OB | 73.15 | / | 10.4 | ** | JHE-like carboxylesterase 1 |
CL1422.Contig1_All | OB | 35.44 | / | 21.85 | ** | Penaeus (Litopenaeus) vannamei microsatellite TUMXLv8.67 sequence |
CL355.Contig1_All | OB | 41.48 | / | 23.46 | ** | Metallothionein |
Unigene12371_All | OB | 67.42 | / | 30.75 | ** | Serine protease-like protein |
Unigene2772_All | OB | 129.19 | / | 52.27 | ** | Crustacyanin-A2 subunit |
Unigene10728_All | OB | 187.39 | / | 57.32 | ** | Putative microtubule-associated protein |
CL160.Contig1_All | OB | 274.18 | / | 58.75 | ** | Mucin-19 |
Unigene9289_All | OB | 188.85 | / | 62.16 | ** | ATP binding cassette transmembrane transporter |
CL1757.Contig1_All | OB | 6.5 | / | 68.51 | ** | Pol-like protein |
Unigene944_All | OB | 232.28 | / | 78.45 | ** | -- |
Unigene2582_All | OB | 82.88 | / | 83.62 | ** | Peroxidase |
Unigene15565_All | OB | 561 | / | 165.67 | ** | PREDICTED: hypothetical protein |
Unigene15673_All | OB | 230.04 | / | 180.28 | ** | Hypothetical protein UY3_06274 |
CL1544.Contig3_All | OB | 2.35 | / | 279.59 | ** | Toll protein |
Unigene7394_All | OB | 186.3 | / | 309.91 | ** | Penaeus monodon progestin membrane receptor component 1 (PGMRC1) |
CL92.Contig2_All | OB | 99.85 | / | 743.69 | ** | Penaeus monodon polehole-like protein Mrna |
Unigene10229_All | OB | 64.19 | / | 754.82 | ** | -- |
Unigene11618_All | OB | 1070.04 | / | 938.62 | ** | -- |
CL92.Contig3_All | OB | 103.95 | / | 1017.81 | ** | Polehole-like protein |
Unigene2102_All | OB | 695.22 | / | 2497.98 | ** | Vitellogenin receptor |
CL989.Contig2_All | OB | 992.51 | / | 3232.49 | ** | Thrombospondin protein |
CL2390.Contig2_All | OB | 226.25 | / | 3345.08 | ** | Cyclin B |
Unigene9017_All | OB | 215.42 | / | 3401.83 | ** | Cyclin B |
CL2487.Contig2_All | OB | 1015.41 | / | 7095.22 | ** | Thrombospondin |
CL1646.Contig1_All | OB | 1025.74 | / | 11552.92 | ** | Thrombospondin-type laminin G domain and EAR repeat-containing protein |
Unigene905_All | OS | / | Not specific | 6.07 | ** | Zebrafish DNA sequence from clone CH211-150Â K4 in linkage group 1 |
CL496.Contig2_All | OS | / | Not specific | 23.23 | ** | Glutamate receptor ionotropic, NMDA 3A |
Unigene7304_All | OS | / | Not specific | 72.23 | ** | X-linked retinitis pigmentosa GTPase regulator [Tupaia chinensis] |
Unigene12972_All | OS | / | Not specific | 117.13 | ** | Peroxidasin |
CL1169.Contig2_All | OS | / | Not specific | 315.15 | ** | PREDICTED: uncharacterized protein |
Unigene12309_All | OS | / | Specific | / | / | Dual oxidase maturation factor 1 |
Unigene8249_All | OS | / | Specific | / | / | PREDICTED: guanine nucleotide-binding protein G(q) subunit alpha-like |
Unigene21442_All | OS | / | Specific | / | / | -- |
Unigene9242_All | OS | / | Specific | / | / | PREDICTED: MD-2-related lipid-recognition protein-like |
Unigene1049_All | OS | / | Specific | / | / | Amyloid beta A4 protein |
CL57.Contig2_All | OS | / | Specific | / | / | Gamma-interferon-inducible lysosomal thiol reductase |
CL711.Contig1_All | OS | / | Specific | / | / | Mucin-5Â AC (Fragments) |
Unigene4288_All | OS | / | Specific | / | / | -- |
Unigene859_All | OS | / | Specific | / | / | -- |