Fig. 5

The identification of ncR40. (a) Schematic representation of ncR40. The termini were examined by small RNA fragments enrichment. The TeloSII elements and TATA box are represented by different boxes: Telo-box (open box), Site II (gray box), and TATA box (black box). The primers designed for ncR40 and their expected sizes are indicated by arrows and solid lines. (b) RT-PCR validation of ncR40 in 2-week-old seedlings. RT+ and RT- refer to the presence and absence of reverse transcriptase, respectively. Molecular markers are shown as M. Genomic DNA, G, was used as a positive control. The expected RT-PCR product size is indicated below the lanes