Table 2 Gene ontology (GO) categories enriched across L. major stages/timepoints. GOseq [101] was used to identify enriched GO categories for the transition between metacyclic promastigotes and 4-h amastigotes and between 4-h amastigotes and 24-h amastigotes at a P value cutoff of < 0.05. For each transition, up- and downregulated genes were considered separately. The category for each enriched GO term is indicated (BP = biological process, MF = molecular function, CC = cellular component). The differentially expressed genes corresponding to each enriched GO category are reported in Additional file 7
GO ID | GO term | P value |
|---|---|---|
Metacyclics to 4-h amastigotes, upregulated | ||
  GO:0006950 | response to stress (BP) | 5.50E-13 |
  GO:0006486 | protein glycosylation (BP) | 9.95E-09 |
  GO:0006457 | protein folding (BP) | 5.70E-06 |
  GO:0051920 | peroxiredoxin activity (MF) | 2.72E-05 |
  GO:0051082 | unfolded protein binding (MF) | 9.44E-05 |
  GO:0016209 | antioxidant activity (MF) | 1.04E-04 |
  GO:0004386 | helicase activity (MF) | 2.06E-04 |
Metacyclics to 4-h amastigotes, downregulated | ||
  GO:0004713 | protein tyrosine kinase activity (MF) | 6.68E-12 |
  GO:0004674 | protein serine/threonine kinase activity (MF) | 2.15E-11 |
  GO:0004672 | protein kinase activity (MF) | 3.40E-10 |
  GO:0006468 | protein phosphorylation (BP) | 3.43E-10 |
  GO:0005516 | calmodulin binding (MF) | 2.76E-08 |
  GO:0009434 | microtubule-based agellum (CC) | 1.93E-07 |
  GO:0005840 | ribosome (CC) | 8.85E-07 |
  GO:0006412 | translation (BP) | 1.42E-06 |
  GO:0003735 | structural constituent of ribosome (MF) | 1.81E-06 |
  GO:0006633 | fatty acid biosynthetic process (BP) | 2.72E-05 |
  GO:0009190 | cyclic nucleotide biosynthetic process (BP) | 8.29E-05 |
  GO:0016849 | phosphorus-oxygen lyase activity (MF) | 8.29E-05 |
  GO:0007165 | signal transduction (BP) | 2.66E-04 |
4-h to 24-h amastigotes, upregulated | ||
  GO:0005874 | microtubule (CC) | 3.25E-09 |
  GO:0043234 | protein complex (CC) | 5.80E-09 |
  GO:0051258 | protein polymerization (BP) | 5.80E-09 |
  GO:0005198 | structural molecule activity (MF) | 3.32E-08 |
  GO:0006184 | GTP catabolic process (BP) | 3.91E-07 |
  GO:0007018 | microtubule-based movement (BP) | 9.88E-06 |
  GO:0003924 | GTPase activity (MF) | 1.81E-05 |
4-h to 24-h amastigotes, downregulated | ||
  GO:0055114 | oxidation-reduction process (BP) | 8.81E-08 |
  GO:0006096 | glycolytic process (BP) | 1.24E-07 |
  GO:0020015 | glycosome (CC) | 4.22E-07 |
  GO:0004743 | pyruvate kinase activity (MF) | 1.50E-05 |
  GO:0050661 | NADP binding (MF) | 2.36E-05 |
  GO:0004365 | GAPDH (NAD+) (phosphorylating) activity (MF) | 5.91E-05 |
  GO:0006006 | glucose metabolic process (BP) | 1.45E-04 |