Fig. 2

Overview of the human 3′UTRome v1 library. a Percentage of cloning success vs 3′UTR length. The efficiency of 3′UTR cloning (blue line) decreases ~30 % beyond the length of 1,000 nucleotides in the cloned dataset (red). The maximum cloning efficiency for amplicons smaller than 499 nt was 92.8 %, while the cloning efficiency of 3′UTRs grater than 2,000nts was 64.3 %. b Length of the 3′UTRs of genes in the human genome hg19 vs the h3′UTRome v1. The 3′UTRs in the h3′UTRome v1 range between 200 nt to 2,500 nt, and are enriched for longer 3′UTR isoforms. The median length of 3′UTRs in the h3′UTRome v1 is 1,159 nt (purple arrow) while in the human genome hg19 it is 1,040 nt (red arrow). c The h3′UTRome v1 contains 3′UTRs for 6-10 % of the ORFs within each chromosome. None of the 3′UTRs cloned in this release originates from the Y chromosome, as the source of genomic DNA used was of female origin. d The degree of overlap between the h3′UTRome v1 and the hORFeome V8.1. More than half of the 3′UTRs cloned in the h3′UTRome v1 contain corresponding clones present in the hORFeome V8.1. e The h3′UTRome v1 (orange circle) is enriched for 3′UTRs of genes involved with gene regulation as it contains predominantly 3′UTRs of transcription factors (33.9 %), kinases (40.8 %), and RNA binding proteins (32.4 %)