The properties of spontaneous mutations in the opportunistic pathogen Pseudomonas aeruginosa

BMC Genomics

Table 3 Patterns of leading/lagging strand mutational bias

		G:C > A:T		A:T > G:C
		G templating the leading strand^a	G templating the lagging strand^a	A templating the leading strand^a	A templating the lagging strand^a
MMR-deficient MA lines	Expected	173	185	166	158
	Observed	279	79	97	227
	Observed Leading/Lagging	3.53		0.43
	Chi-square value	67.42		30.47
	p value	<0.0001		<0.0001
Wild-type MA lines	Expected	12	13	4	4
	Observed	15	10	6	2
	Observed Leading/Lagging	1.50		3.00
	Chi-square value	0.73		1.07
	p value	>0.39		>0.30
Comparative genomics of clinical isolates	Expected	16364	16384	8509	8511
	Observed	17794	14954	8837	8183
	Observed Leading/Lagging	1.19		1.08
	Chi-square value	125.12		12.65
	p value	<0.0001		<0.0004

Observed values that are greater then expected are shown in bold. Data from both replichores were combined
^aFunctionally equivalent transition types were combined. For example, G templating the leading strand in the right replichore is functionally equivalent to C templating the lagging strand in the left replichore

ISSN: 1471-2164