Removal of redundant contigs from de novo RNA-Seq assemblies via homology search improves accurate detection of differentially expressed genes

Table 2 Summary of gene dataset and reference contigs derived from de novo assembly of RNA-Seq reads in A. thaliana

	Gene dataset	Raw Contigs	Longest Contigs	Clustered Contigs	Annotated Contigs	Plant DB Contigs	PlantClust50 DB Contigs
Number	35,385	62,339	58,007	59,405	23,873	42,467	26,766
Min. length (base)	22	121	121	121	121	121	121
Median length (base)	1383	285	275	281	599	317	351
N50 (base)	1814	739	699	711	1042	826	987
Mean length (base)	1535	475	457	464	757	520	589

ISSN: 1471-2164