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Table 3 Frequencies of CNVs in experimental C. elegans lines at different time intervals of population expansion using single-worm PCR

From: Rapid Increase in frequency of gene copy-number variants during experimental evolution in Caenorhabditis elegans

Pop ID. generation

Type of rearrangements

Individuals sampled

Number positive for rearrangement

Expectec Hardy-Weinberg frequency

Average copy-number

qPCR

oaCGH

16B.80

Duplication

19

0

0.00

1.00

-

-

16B.140

47

0

0.00

1.00

0.86

-

16B.200

43

28

0.41

1.41

1.02

1.19

16E.80

Duplication

30

1

0.02

1.02

1.08

-

16E.140

30

25

0.59

1.59

1.58

-

16E.200

18

18

1.00

2.00

2.39

2.08

66E.140

Duplication

27

10

0.20

1.20

1.23

-

66E.200

28

22

0.54

1.54

1.67

1.33

16A.140

Deletion

30

30

1.00

0.00

0.00

-

16A.200

27

27

1.00

0.00

0.00

0.05

16D.80

Deletion

18

15

0.64

0.36

0.22

-

16D.140

18

28

1.00

0.00

0.000232

-

16D.200

29

29

1.00

0.00

0.000738

0.05

66B.80

Deletion

32

0

0.00

1.00

0.73

-

66B.140

15

9

0.40

0.60

0.40

-

66B.200

28

28

1.00

0.00

0.0000269

0.04

  1. Pop ID.Generation refers to the experimental population number and the number of generations of population expansion when sampled. Column 5 displays the frequency of individuals with the rearrangement assuming Hardy-Weinberg equilibrium. Columns 6–8 show the average copy-number per haploid genome for three methods, namely single-worm PCR, qPCR and oaCGH