Fig. 4

Analysis of the two different transcript isoforms of TRAES_1DS_114C78BF4 by RT-PCR. a A diagram showing the exon (filled box)-intron (line in between filled boxes) patterns of the two isoforms (designated as a and b, respectively). Arrows mark the positions of the primers (FP, RP1 and RP2) used for specifically amplifying each of the two isoforms. The length of the amplicon (bp) is indicated for each isoform. b The result of amplifying isoforms a and b by RT-PCR in the caryopsis samples of four developmental stages (S1 - S4). Amplification of the common wheat actin gene (GenBank accession AB181991) served as internal control for normalizing cDNA content prior to PCR amplification. S1, unfertilized caryopses; S2-S4, developing grains collected at 5, 15 or 25 DAA. The data displayed are typical of three independent experiments