Single-molecule real-time transcript sequencing facilitates common wheat genome annotation and grain transcriptome research

Table 2 Estimation of the genes expressed in unfertilized caryopses and developing grains

	Developmental stage^a
	S1	S2	S3	S4
Total number of genes expressed^b	50,650	42,444	44,547	37,369
Number of genes with coverage by PacBio sequencing identified transcripts	11,798	9452	10,626	8676
Total number of PacBio sequencing identified transcripts detected at each stage	17,330	13,938	15,909	12,943

^aS1, unfertilized caryopses; S2-S4, developing grains at 5, 15 or 25 days after anthesis
^bJudged based on RPKM (reads per kilobase per million mapped reads) > 1

ISSN: 1471-2164