TY - JOUR AU - Li, Wenli AU - Turner, Amy AU - Aggarwal, Praful AU - Matter, Andrea AU - Storvick, Erin AU - Arnett, Donna K. AU - Broeckel, Ulrich PY - 2015 DA - 2015/12/16 TI - Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysis JO - BMC Genomics SP - 1069 VL - 16 IS - 1 AB - Whole transcriptome sequencing (RNA-seq) represents a powerful approach for whole transcriptome gene expression analysis. However, RNA-seq carries a few limitations, e.g., the requirement of a significant amount of input RNA and complications led by non-specific mapping of short reads. The Ion AmpliSeqâ„¢ Transcriptome Human Gene Expression Kit (AmpliSeq) was recently introduced by Life Technologies as a whole-transcriptome, targeted gene quantification kit to overcome these limitations of RNA-seq. To assess the performance of this new methodology, we performed a comprehensive comparison of AmpliSeq with RNA-seq using two well-established next-generation sequencing platforms (Illumina HiSeq and Ion Torrent Proton). We analyzed standard reference RNA samples and RNA samples obtained from human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs). SN - 1471-2164 UR - https://doi.org/10.1186/s12864-015-2270-1 DO - 10.1186/s12864-015-2270-1 ID - Li2015 ER -