Fig. 5From: Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysisAmpliSeq is robust in identifying significantly differentially expressed genes between UHRR and HBRR using the ARBF PrimePCR as the gold-standard. For genes in the two bottom quartiles, AmpliSeq performs comparably to the two RNA-seq methods. The average AUC values for AmpliSeq, Illumina RNA-seq and Proton RNA-seq are 0.95, 0.96 and 0.95 respectively. For genes in the top two quartiles, AmpliSeq performed better than the two RNA-seq methods with an average AUC value of 0.95 while Illumina and Proton RNA-seq have an average of 0.81 and 0.84 respectivelyBack to article page