Fig. 4

Expression of ISA1307 genomic library plasmids containing a single ORF in S. cerevisiae mutants deleted for the corresponding homologous gene. Growth curves of S. cerevisiae mutants gyp8Δ, ktr7Δ, rkr1Δ, msn4Δ, pmt1Δ, wsc4Δ, tif3Δ and ilv3Δ transformed with the corresponding plasmid from the strain ISA1307 genomic library, specifically 8.23, S07, Y08, B02, 18.22, 23.1, B18 and 23.17, respectively, which contained the putative ISA1307 homologue of the S. cerevisiae gene deleted in each mutant (■) or with the empty vector (□). Strains were cultivated in MM4 medium (pH 4.0) without uracil or in this same basal medium supplemented with 60 mM (a), 40 mM (b) and 65 mM (c) of acetic acid. S. cerevisiae BY4741 and BY4743 parental strains were also transformed with the empty vector (○) for control purposes. The growth curves shown are representative of three independent assays