Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium

BMC Genomics

Table 1 Comparison of the discrepancies occurring between the current and Brown et al. whole genome sequencing of C. autoethanogenum

Position	Insertion	Gene	Homopolymer length		Amino acid length		Sequence identity
			CLAU	CLJU	CLAU	BRO	BRO	CAUT	CLJU
46129	T	CAETHG_0051	6	6	412	412	119/367	NF	412/412
283331	C	CAETHG_0263	5	5	370	370^a	NF	370/370	369/370
627984	C	CAETHG_0567	2	2	521	245	231/233	NF	521/521
656810	T	CAETHG_0595	6	6	722	279	269/269	722/722	717/722
928129	C	CAETHG_0862	5	5	293	250	249/249	NF	293/293
985484	C	CAETHG_0915	4	4	688	688	NF	NF	688/688
1106176	A	CAETHG_1030	6	6	172	126	109/109	NF	172/172
1457002	C	CAETHG_1363	6	6	296	254	249/249	294/295	292/296
1603900	T	CAETHG_1501	8	8	401	401	NF	NF	401/401
1620246	T	CAETHG_1521	6	NF	323	316	315/315	323/323	310/323
2222019	T	CAETHG_2078	8	8	445	326	325/325	NF	444/445
2352969	T	CAETHG_2212, CAETHG_2213	2	2	416	202	None	416/416	414/416
2596835	G	CAETHG_2429	7	7	400	382	378/378	400/400	400/400
2683087	C	CAETHG_2503	4	4	640	615	601/605	640/640	639/640
2805023	A	CAETHG_2601, CAETHG_2602	7	AAAGAAA	370	141	138/138	370/370	328/366
2852812	T	CAETHG_2647	8	NF	470	314	314/314	469/470	NF
3076804	A	CAETHG_2840	8	8	635	487	482/483	635/635	635/635
3396986	G	CAETHG_3132, CAETHG_3133	5	5	160	152	149/149	160/160	160/160
3468796	G	CAETHG_3212	5	5	271	291	270/271	270/271	270/271
3752592	G	CAETHG_3500	5	5	459	418	413/415	459/459	459/459
3786709	T	CAETHG_3531	6	NF	144	64	64/64	144/144	NF
3877937	A	CAETHG_3599	3	3	270	74	181/182	270/270	269/270
3994749	G	CAETHG_3707	6	6	261	176	172/177	NF	261/261
4180142	T	CAETHG_3902	5	5	359	99	94/95	NF	359/359

This table shows a representation of the discrepancies that occur when the current Illumina sequence (CLAU) is mapped against the published Brown et al. sequence (BRO). The insertion column describes the mutation occurring in the CLAU genome compared to the BRO genome. Homopolymer length indicates the number of the same base occurring consecutively at the site of the discrepancy. Amino acid length gives the annotated protein length of the gene in which the discrepancy occurs. The sequence identity is relative to our C. autoethanogenum genome sequence when protein BLAST searched on the NCBI database. CLAU, C. autoethanogenum finished genome sequence in present study; CLJU, C. ljungdahlii DSM 13528 finished genome sequence (GCA_000143685.1); BRO, Brown et al. C. autoethanogenum finished genome sequence (GCA_000484505.1); CAUT, Bruno-Barcena et al. C. autoethanogenum draft genome sequence (GCA_000427255.1); NF not found. ^aindicates protein codes for multiple stop codons

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