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Fig. 3 | BMC Genomics

Fig. 3

From: Genome-wide analysis, molecular cloning and expression profiling reveal tissue-specifically expressed, feedback-regulated, stress-responsive and alternatively spliced novel genes involved in gibberellin metabolism in Salvia miltiorrhiza

Fig. 3

Phylogenetic analysis of GA20ox proteins and expression profiling of SmGA20ox genes. a: Phylogentic relationship of GA20ox proteins in Arabidopsis thaliana (At), Lactuca sativa (Ls), Lolium perenne (Lp), Nicotiana tabacum (Nt), Oryza sativa (Os), Phaseolus vulgaris (Pv), Pisum sativum (Ps), Salvia miltiorrhiza (Sm), Solanum dulcamara (Sd), Solanum lycopersicum (Sl), and Spinacia oleracea (So). The unrooted neighbor-joining tree was constructed using the MEGA 6.0 [64]. SmGA20ox proteins from S. miltiorrhiza are in bold. Clades 1–4 indicate the four clades identified. b–g: Fold changes of SmGA20ox1 (b), SmGA20ox2 (c), SmGA20ox3 (d), SmGA20ox4 (e), SmGA20ox5 (f) and SmGA20ox6 (g) in roots (Rt), stems (St), leaves (Le) and flowers (Fl) of S. miltiorrhiza plants. The expression levels were analyzed using the quantitative RT-PCR method. Expression level in leaves was arbitrarily set to 1 and the levels in other tissues were given relative to this. Error bars represent standard deviations of mean value from three biological and four technical replicates. ANOVA (analysis of variance) was calculated using SPSS. P < 0.05 was considered statistically significant

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BMC Genomics

ISSN: 1471-2164

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