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Table 4 Putative C. coli BFR-CA-9557 restriction modification systems

From: SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs

ORF #1

Strand

Position in genome

Description

Type/subunit

Predicted rec. seq.

465

+

86828-89146

hsdR, Type I restriction-modification system2C restriction subunit R (EC 3.1.21.3)

I/R

-

485

+

92205-93437

hsdS, Type I restriction-modification system2C specificity subunit S (EC 3.1.21.3), EcoKI specificity protein

I/S

-

495

+

94709-96196

Type I restriction-modification system2C DNA-methyltransferase subunit M (EC 2.1.1.72)

I/M

-

6540

-

1246404-1250480

Type I restriction-modification system2C DNA-methyltransferase subunit M (EC 2.1.1.72) / Type I restriction-modification system2C specificity subunit S (EC 3.1.21.3)

I/MS

(GAGNNNNNGT)3,4

720

+

139123-140043

fokIM_1, Modification methylase FokI, (EC 2.1.1.72), homologue to ulcer associated adenine specific DNA methyltransferase

II/M

(GGATG)3

1470

-

275768-271959

Type IIS restriction enzyme Eco57I

II/S

-

2605

+

488272-489375

DNA modification methylase (Adenine-specific methyltransferase), fokIM_2, Modification methylase FokI, (EC 2.1.1.72)

II/M

GAATTC

2895

+

541751-542602

DNA modification methyltransferase, dpnA, Modification methylase DpnIIB (EC 2.1.1.72)

II/M

GATC

6520

-

1240865-1243951

N-6 adenine specific DNA methyltransferase

II/M

-

7730

+

1464470-1465285

DNA adenine methylase, dpnM, Modification methylase DpnIIA, EC 2.1.1.72

II/M

-

8910

+

1701685-1702374

16S rRNA (guanine(966)-N(2))-methyltransferase (EC 2.1.1.171)

II/M

GATC5

2225

+

409683-409970

McrBC 5-methylcytosine-specific restriction endonuclease system2C McrB subunit2C putative

IV/R

-

22302

+

409982-411148

McrBC 5-methylcytosine-specific restriction endonuclease system2C McrB_1 subunit2C putative

IV/R

-

22302

+

411208-412158

McrBC 5-methylcytosine-specific restriction endonuclease system2C McrB_2 subunit2C putative

IV/R

-

2235

+

412124-413494

McrBC 5-methylcytosine-specific restriction enzyme subunit McrC

IV/R

-

  1. 1ORF # according to the NCBI annotation pipeline for RAST and Prodigal ORF # see Additional files 3 and 4
  2. 2disrupted ORF
  3. 3predicted sequence does not correspond to any motif detected by SMRT sequencing
  4. 4According to REBASE ORF #6540 encodes a Type II RM-system
  5. 5In contrast to RAST and Prodigal REBASE predicts a second candidate for a GATC-specific DNA modification methyltransferase for this ORF
  6. Restriction modification systems have been identified as outlined in section “Methods”. Column 1 to 3 denote the number, strand direction and genome position of the ORF as identified by the NCBI annotation pipeline. Column 4 contains the description of the ORF in terms of aggregated annotations from NCBI, RAST and Prodigal. The type and predicted recognition sequence of the motif are shown in columns 5 and 6