Fig. 2
Differential gene expression detected by chromosome mapping. a Chromosome mapping of gene expression fold differences between FCB and FCBpfmdr1_3′KD shows differential transcription of a large fragment on chromosome 5 corresponding to the pfmdr1-containing 100-kb amplicon region. For each gene located on chromosome 5, the expression fold difference between FCB and FCBpfmdr1_3′KD was mapped using the chromosomal coordinates. Interestingly, PF3D7_0507500 (a subtilisin-like protease) and PF3D7_0501200 (a parasite-infected erythrocyte surface protein) show higher expression in FCB in comparison to both FCBpfmdr1_3′KD and 106/1 (see panel B). The inset shows the genes within the pfmdr1-containing amplicon region. b Chromosome mapping of gene expression fold differences between FCB and 106/1 for each gene on chromosome 5 with the inset showing genes within the pfmdr1-containing amplicon region. c Chromosome mapping of gene expression fold differences between 7G8 and 7G8pfcrt_T76K shows differential gene expression of a large number of genes located on chromosome 2 (inset shows a cluster of genes coding for proteins involved in parasite invasion). d Chromosome mapping of gene expression fold differences between 7G8 and 7G8pfcrt_CTL for each gene on chromosome 2. In each pairwise comparison, the fold difference in gene expression was calculated as the difference between the areas under the curve computed on the full 384 time point data