Fig. 8
Investigation of pfcrt and pfmdr1 expression networks: Gene expression correlations between all P. falciparum genes and pfcrt/pfmdr1. a Cluster heatmap of expression data for P. falciparum genes. The hierarchical clustering was generated using Pearson correlation coefficients (PCC) calculated using log2-transformed and normalized expression values of 2,600 genes across 55 pairwise comparisons of 11 parasite transcriptome data sets. Heatmaps of the hierarchical clustering show several domains of high correlation between gene pairs, including a large cluster corresponding to strong interactions between plasmepsin X (PF3D7_0808200) and multiple genes involved in cell motility such as myosin (PF3D7_1246400), kinesin-like protein (PF3D7_0724900) and invasion like rhoptry (PF3D7_0414900) or IMC1-related (PF3D7_0304100) proteins. Two smaller clusters (boxes 1 and 2) are shown including one containing both pfcrt and pfmdr1. b Venn diagrams showing the number of genes whose expression highly correlates (PCC >0.7) (top) or anti-correlates (PCC <−0.7) (bottom) with that of both pfcrt and pfmdr1. PCC values were calculated using log2-transformed and normalized expression values of 2600 genes across 110 pairwise comparisons of 11 parasite transcriptome data sets