Fig. 8

XT4699-Tal6 effector modulates gene expression in wheat. First leaves of 10-day-old Chinese Spring wheat plants were inoculated with XT4699, M2, M2 (empty vector, ev for short) and M2 (tal6), respectively. Inoculated leaves were collected 24 h post inoculation. Gene specific primers were used for qRT-PCR in each panel. Relative expressions of Ta.7291.1.S1_s_at (a and c) and Ta.14164.1.S1_s_at (b and d) were calculated; In (a) and (b), the fold change relative to M2 (ev) treatment was calculated with 2^-∆∆Ct method. In (c) and (d), bacterial strains treated with 100 μm cycloheximide (CHX) were applied for inoculation. The fold change relative to hrcC ⁻ + CHX treatment was calculated with 2^-∆∆Ct method. The lowercase letters indicate significantly different groups with P-value < 0.05 in the ANOVA statistics analysis. Two biological replicates were applied, and the experiments were repeated twice with consistent results