Skip to main content

Table 1 Primers, plasmids, and strains used in the mouse intranasal challenge model experiments

From: Using host-pathogen protein interactions to identify and characterize Francisella tularensis virulence factors

Primer, plasmid, or strain

Relevant characteristics

Source or reference

Primer

  

FTT0482d5’

GCTCAACATTGTATGAAATTAATGGCTCCA

Invitrogen

FTT0482p5’

GTGAAATAGTCAGACAAGTAAGCCTTGGT

Invitrogen

FTT0482d3’

ATTTGTATCAGCCAAATGCTGTTACGCA

Invitrogen

FTT0482p3’

GTAGAATGTGGATGAATGTTAAGTACGGT

Invitrogen

FTT0902d5’

CTGCTGCTGCTCAGACAGCTACTACTG

Invitrogen

FTT0902p5’

GCTGCTGCTGTATCTAAGCCAACTGC

Invitrogen

FTT1538d5’

CAGCAGGCGATTATGGCTACAAACA

Invitrogen

FTT1538p5’

GCAGGTCAGATGTCGACACAAGAAGC

Invitrogen

FTT1538d3’

TGCTTCAGCTTCGGACTTAGCAACA

Invitrogen

FTT1538p3’

ATCCAATTGCTGCATCCACACCATCA

Invitrogen

FTT1564d5’

CTCCTCCTCATATCAGTTCTGTCAGCT

Invitrogen

FTT1564p5’

GTCGGTTTCCCAAGCTACTGGAATGT

Invitrogen

FTT1564d3’

GGACTCGAACCTACGACCTACGGAT

Invitrogen

FTT1564p3’

CTAACCAACTGAGCTATAGGCCCA

Invitrogen

FTT1597d5’

CGGCTGATAATGATGGCTTTATGGCT

Invitrogen

FTT1597p5’

TGATCCTCCTGAATATGATGATCCTAGT

Invitrogen

FTT1597d3’

GCATATACGGCTGAATCTTGCCACCT

Invitrogen

FTT1597p3’

CAATGATGCCAATGCCGCGGTAACT

Invitrogen

Plasmid

  

pCR2.1-TOPO

3,931-bp TA vector; pMB1 oriR; Kmr

Life Technologies

pGreenhopper

pCR2.1-TOPO containing a 4-kbp Mariner-Himar 1 transposon with kanamycin resistance gene and a 1.2 kbp tnpA transposition gene. Non-replicable in F. tularensis

Unpublished

E. coli

  

TOP10

General cloning and blue/white screening

Life Technologies

F. tularensis

  

FT12

Type strain, a mouse-passaged isolate of a strain obtained from a U.S. patient

McKesson BioServices