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Fig. 5 | BMC Genomics

Fig. 5

From: Comparison of normalization and differential expression analyses using RNA-Seq data from 726 individual Drosophila melanogaster

Fig. 5

Comparison of the number and identity of differentially expressed genes obtained using the generalized linear model (GLM) with those obtained using the ANOVA model. The graph shows the percentage of differentially expressed genes for the Genotype × Environment × Sex term that agree between the GLM and ANOVA methods. Dark blue bars, overlap of DESeq GLM and ANOVA for Workflow 1; Light blue bars, overlap of DESeq GLM and ANOVA for Workflow 2; Medium blue bars, overlap of DESeq GLM and ANOVA for Workflow 3; Dark red bars, overlap of edgeR GLM and ANOVA for Workflow 1; Light pink bars, overlap of edgeR GLM and ANOVA for Workflow 2; Medium pink bars, overlap of edgeR GLM and ANOVA for Workflow 3. Abbreviations for normalization methods are the same as defined in Fig. 1

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