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Fig. 1 | BMC Genomics

Fig. 1

From: Improved transcription and translation with L-leucine stimulation of mTORC1 in Roberts syndrome

Fig. 1

Ribosomal components and translation initiation complexes were present at low levels in RBS cells. a Western blotting showed that 40S small ribosome proteins RPS7 and RPS19, and 60S large ribosome proteins RPL5, RPL23, and RPL24 were decreased in ESCO2 mutant (M) compared to WT fibroblasts (WT) or corrected fibroblasts (C). b L-Leu supplement, but not D-Leu, partially rescued RPS7 and RPL24 protein levels, and reversed the elevation of eIF2α phosphorylation in RBS cells. α-Tubulin and eiF2α serve as loading controls. c m7-GTP coupled beads were used to pull down translation initiation complexes from whole cell lysates. 4EBP1 protein was strongly enriched in RBS cells, accompanied by less binding of eIF4G1, but this trend was partially reversed in RBS cells treated with L-Leu. eIF4E levels were not affected. d Antibodies to eIF4E were used to pull down translation initiation complexes. 4EBP1 was present at high levels in RBS cells, correlating with less eIF4G1 and the inhibition of translation initiation. L-Leu supplement promoted the assembly of the translation competent eIF4E complex. e Antibodies to eIF3B were used to pull down translation initiation complexes. eIF4E and eIF4G1 were present at lower levels in RBS cells, but this trend was partially reversed by L-Leu supplement

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