Fig. 2

Phylogenetic analysis of Bcl-2 proteins identified in A. millepora. The phylogenetic tree shown was calculated using a Bayesian approach (MrBayes) based on a ClustalW alignment of Bcl-2 domains (for detailed information, see Materials and Methods). The dataset was also subjected to Maximum Likelihood analysis (Additional file 1). Posterior probability values are shown at each node, but at critical nodes, both posterior probability (black) and bootstrap support (red) data are shown as X / Y. Details of protein sequences are given as Additional file 7. Branch lengths are proportional to the number of substitutions per site in the Bayesian analysis (see scale bar in the figure). Background shading is used to emphasize relatedness of cnidarian and/or vertebrate proteins. Red backgrounds underlie well-supported clades consisting of both cnidarian and vertebrate sequences; note that the vertebrate members of these clades are exclusively pro-apoptotic. Blue or green backgrounds underlie well-supported clades consisting exclusively of vertebrate or cnidarian sequences respectively. Acropora millepora sequences are printed bold