Fig. 6

A209T as a compensatory substitution for Q214H. a The result from coevolution analysis on M1 protein using CAPS [50] is shown as a network. Each node represents a residue and is labeled with the amino acid position. Nodes representing residue on N-terminal domain (residues 1–164) are in rectangular shape. Nodes representing residue on C-terminal domain (residues 165–252) are in eclipse shape. An edge is drawn between coevolving residues. Residues 121, 207, 209, and 214 were identified as a coevolving group by CAPS [50] and are highlighted in cyan. b The TCID₅₀ measured from the virus rescue experiment for the wild-type (WT) or the indicated mutant is shown. This data represent the mean value from three independent replicates. The grey dashed line represents the lower detection limit. c A multicycle replication assay was performed. A549 cells were infected with wild-type (WT) or the indicated mutant at an MOI of 0.005. Virus was harvested at the indicated timepoints and the TCID₅₀ was measured