Fig. 3

Quantitative real-time PCR confirms transcript changes in diapausing flies. Estimate of relative gene expression, comparing (ΔΔCt) three week old diapausing flies (3wD) with three week old flies kept under normal conditions (3wN). Directionality and relative magnitude of change matches microarray data in all cases, except Vm32E. We used Welch’s unpaired t-test with corrections for non-equal variances, comparing expression at 3wD and 3wN for each gene, ns, not significant; *, p < 0.05; **, p < 0.01. Error bars show standard error of the mean, n = 4. Acronyms used: FAD2 - Fatty Acid Desaturase 2, HS67Bc - Heat Shock 67 Bc, LSPgamma - Larval Serum Protein gamma, NeurpepRecF - Neuropeptide receptor F, OBP19b - Odorant Binding Protein 19 b, Per – Period, Tim-1 - Timeless, (targets all Timeless isoforms except N and O), Tim-2 - Timeless, (targets N and O isoforms of Timeless), Tim-Total - Timeless, (targets all Timeless isoforms), VME32e - Vitelline Membrane 32e