Fig. 2
From: FabR regulates Salmonella biofilm formation via its direct target FabB
ChIP-qPCR validation of the ChIP-chip data. The validation of the ChIP-chip results by ChIP-qPCR analysis was performed as elaborated in Materials & Methods with dnaG as endogenous control. Values represent enrichment ratios of ChIP over mock ChIP samples, generated under free-living TSB conditions, and are averages of triplicate ChIP-qPCRs. The enrichment ratios of ChIP over mock ChIP samples were calculated as RQ = 2-(∆Ct ChIP -∆Ct mock ChIP), in which ∆CtChIP is Ctgene test – Ct dnaG for the ChIP samples and ∆Ctmock ChIP is Ctgene test – Ct dnaG for the mock ChIP samples. The presented values are representative for two biological repeats and standard deviations of the three technical repeats are indicated