Table 1 Bacterial strains and plasmids

Strains

E. coli DH5α

F⁻ ϕ80ΔlacZM15 Δ(lacZYAargF)U169 deoP recA1 endA1 hsdR17 (r_k ⁻ m_k ⁻) λ⁻

Gibco BRL

E. coli TOP10F’

F’ {lacIq Tn10(TetR)} mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG

Invitrogen

S. Typhimurium SL1344

Wildtype strain, xyl hisG rpsL; virulent; Sm^R

[82]

CMPG5624

S. Typhimurium SL1344, ΔfabR

This study

CMPG5825

S. Typhimurium SL1344, fabR-M9

This study

Plasmids

c3390

SpeI-myc9-SpeI cassette in pBlueskript SKII-

[39]

pCP20

flp, ts-rep-[ciI857](λ) ts, Ap^R, Cm^R

[38]

pCMPG5553

yqfA cloned XbaI/SacI in pFAJ1708

This study

pCMPG5678

fabR cloned EcoRI/BamHI in pFAJ1708

This study

pCMPG10118

fabA cloned XbaI/EcoRI in pFAJ1708

This study

pCMPG10119

fabB cloned XbaI/EcoRI in pFAJ1708

This study

pFPV25

Promoter-trap vector constructed by inserting an EcoRI-HindIII fragment containing a promoterless gfpmut3 [83] into plasmid pED350 (colE1, bla, mob) [84]; ApR

[85]

pKD3

Plasmid used as template for construction of Salmonella mutants; Ap^R, Cm^R

[38]

pKD46

Lambda Red helper plasmid, Ap^R

[38]