Fig. 5

NetGenerator derived model prediction of MPT and FPT cascade. a-b Interpolated expression (dotted line; dots indicate actual recorded fold change) and inferred patterns derived from the predicted NetGenerator model (solid line) of putative transcriptional regulatory genes over the course of sex differentiation at both (a) MPT and (b) FPT. c-d Inferred network model at both (c) MPT and (d) FPT. Robust (solid line) and non-robust (dotted line) predicted interactions are displayed as gene-gene interactions (black), and temperature-gene interactions (MPT:blue; FPT:red). Thickened lines indicate interaction with high robustness (80 % or above). Following genes names were modified for inference network: LOC102562106 (JARID2-1), LOC102561337 (JARID2-2), LOC102569158 (WNT11), LOC102560544 (EEF1A1), LOC102577358 (DMRT3), LOC102573123 (SOX9), LOC102576325 (PAK1), LOC102559361 (EDNRB), LOC102563625 (PDE2A), and LOC102577040 (FOXL2)