Fig. 6

Exploratory data analysis of differentially expressed genes in SBT gonad cells across all comparison groups. a – Cluster analysis of SBT gonad samples. Red color in matrix represents no difference between samples and green represents maximum distance; Colored bars on top and left of figure represent one treatment group each: green for enriched ovary cells (labeled SBT_OC), red for crude ovary cells (labeled SBT_O), light blue for testis cells (labeled SBT_T) and purple for Percoll-enriched testis cells (labeled SBT_TP). b – Principal component analysis (PCA) of regularized log-transformed counts in all samples to identify the effects of germ cell enrichment treatment and gonad type (testis or ovary). c – PCA of regularized log-transformed counts in testis samples to identify the effects of the Percoll enrichment treatment and individual fish across samples. d – PCA of regularized log transformed counts in the ovary samples to identify the effect of the filtration enrichment treatment and individual fish across samples