Fig. 1From: A multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymesSchematic representation of the methodology used to screen for (hemi) cellulases in fosmid-based metagenomic libraries. 1) Biased communities (e.g. soil-derived microbial consortia cultivated on wheat straw) are at the basis of enhanced hit rates. 2) Screening of fosmid pools (700 fosmids per pool) allow high-throughput analyses. 3) Screening on substrate mixtures accelerate the analyses. 4) Re-screening of positive clones in single, double and further mixed combinations of substrates enable the detection of specific activitiesBack to article page