Fig. 4

Transient targets initiated by a bZIP1 “hit” are actively transcribed after the TF has “run”. a-b bZIP1 mediates rapid and catalytic transcription in response to nitrogen signal. a Time-series ChIP-Seq binding 1 kb upstream and downstream of the transcription start site (TSS) for bZIP1 transient targets, NLP3, THA1 and MCCA at 1 and 5 min (early time-points), 30 min, 1 and 5 h (late time-points) after induced nuclear localization of bZIP1. The bZIP-binding motifs identified by known motif enrichment (also see Fig. 2) were placed on the x-axis of early time-points (2 ACGT boxes in NLP3 promoter at −826 and -584 bp, and 1 box in MCCA promoter at -126 bp). The bZIP1 ChIP-Seq time-course analyzed in this study was initially performed in [6]. b Gene expression levels of transient bZIP1 targets NLP3, THA1 and MCCA in 4tU-labeled fractions of bZIP1 expressing cells (4tU-bZIP1) compared to 4tU-labeled fractions of empty vector (4tU-EV; *FDR < 0.1). Please note that the ChIP-Seq time course (a) and de novo transcriptomics (b) data were performed in two independent experiments. c “Hit and Run” mode of transcription for transient TF-targets