Fig. 1

Cell morphology and dynamic changes in abundance of histone PTMs. a Retinoic acid differentiation of ESCs results in neuronal cells. From top to bottom, CCE ESCs were grown in suspension for 4 days in the absence of LIF/2i to form embryoid bodies. The embryoid bodies were treated with 1 μM all-trans retinoic acid for 4 days. Cells were plated on gelatin-coated plates and grown for 4 days and 7 days. Red scale bar represents 100 μm. b Relative abundance of histone PTMs during cell differentiation. Heatmap of all single histone modifications from histone H3 and H4, calculated by summing the relative abundance of all peptides carrying each given PTM. A and B represent the two biological replicates for each analyzed time point. The represented value was obtained by averaging the three technical replicates and performing z-score normalization for the rows