Table 1 The R. clarus effector candidates with predicted function
From: The effector candidate repertoire of the arbuscular mycorrhizal fungus Rhizophagus clarus
Predicted effector | Length (aa) | Similarity % | Homology to (e-value/homology lenght) | Conserved domains | Cellular role | Putative function |
|---|---|---|---|---|---|---|
Interference with signal transduction | ||||||
NLS_26232 | 592 | 91 | Salt-inducible protein kinase (NP_001105276.1) Zea mays 4.43e−26/259) | Protein kinase-like, PKc (7.5e−34) | Cell envelope | Interference with signal transduction |
NLS_30765 | 542 | 92 | No significant hits | F-box-like domain (2.8e−07) | Cell envelope | Interference with signal transduction |
NLS_334409 | 614 | 95 | Nitrogen permease regulator 3-like protein isoform X1 (XP_004596640.1) Ochotona princeps (9e−47/595) | Nitrogen Permease regulator of amino acid transport activity 3 (9e−88) | Cell envelope | Interference with signal transduction |
NLS_320155 | 274 | 89 | PLC-like phosphodiesterase (EJF62321.1) Dichomitus squalens (2e−41/256) | Glycerophosphodiester phosphodiesterase, GDPD (3.0e−53) | Cell envelope | Interference with signal transduction |
RCP_340423 | 175 | 88 | Phospholipase d1 (EFX04791.1) Grosmannia clavigera (7e−07/98) |  | Cell envelope | Interference with signal transduction |
SCR_348911 | 85 | 53 | Peptidoglycan-binding LysM (YP_001663206.1) Thermoanaerobacter sp. X514 (2e−07/53) | LysM domain (3.5e−12) | Cell envelope | Interference with signal transduction |
NLS_7749 | 827 | 97 | Trehalose phosphate synthase (EKD17284.1) Marssonina brunnea (0.0e/790) | Bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB domain (0.0e) | Cell envelope | Interference with signal transduction |
SCR_343180 | 150 | 85 | No significant hits | ML (MD-2-related lipid-recognition) (2e−07) | Cell envelope | Interference with signal transduction |
Cell wall modification | ||||||
NLS/RCP_349824 | 610 | 83 | Galactose oxidase (CCO36399.1) Rhizoctonia solani (1.8e−70/549) | Glyoxal oxidase N-terminus domain (1e−29) | Cell envelope | Plant cell wall modification |
RCP_230436 | 569 | 89 | class I alpha-mannosidase protein (EOD46381.1) Neofusicoccum parvum (1.8e−134/523) | Glycosyl hydrolase family 47 (0.0e) | Cell envelope | Fungal cell wall modification |
RCP_343321 | 399 | 90 | Chitin deacetylase (XP_003194854.1) Cryptococcus gattii (3e−64/289) | Chitin deacetylase domain (1.4e−33) | Cell envelope | Fungal cell wall modification |
RCP_84949 | 326 | 70 | Bifunctional xylanase/deacetylase (CCO26450) Rhizoctonia solani (4e−55/246) | Catalytic NodB homology domain of the carbohydrate esterase 4 superfamily (1.8e−87) | Cell envelope | Fungal cell wall modification |
Hevein/Chitin binding domain (3.8e−10) | ||||||
Transcription regulation | ||||||
NLS_98735 | 263 | 95 | Hypothetical protein (EIE87396.1) Rhizopus delemar (8e−13/105) | PWWP domain (1.5e−18) | Transport and binding | Transcription regulation |
Unknown function | ||||||
NLS_32583 | 730 | 91 | DENN domain containing protein (EJY85661.1) Oxytricha trifallax (1e−49/498) | ATPase family associated with various cellular activities (AAA) (1.5e−05) | Amino acid biosynthesis |  |
NLS_343100 | 654 | 96 | P-loop containing nucleoside triphosphate hydrolase protein (EIW63580.1) Trametes versicolor (5e−34/394) |  | Cell envelope |  |
SCR_339199 | 125 | 74 | Carbohydrate-binding module family 19 protein (XP_001874952.1) Laccaria bicolor (4e−13/88) |  | Transport and binding |  |
SCR_94594 | 137 | 79 | Proline-rich protein (XP_001875220.1) Laccaria bicolor (8e−23/ 78) |  | Cell envelope |  |
RCP_349288 | 473 | 61 | Carbohydrate-binding module family 19 protein (XP_001874952.1) Laccaria bicolor (9e−17/81) |  | Cell envelope |  |
RCP_335225 | 347 | 84 | Hypothetical protein (EPZ34955) Rozella allomycis (2e−72/315) |  | Cell envelope |  |