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Table 2 Classification of expressed genes according to level of expression

From: Transcriptome adaptation of the bovine mammary gland to diets rich in unsaturated fatty acids shows greater impact of linseed oil over safflower oil on gene expression and metabolic pathways

Category

aRPKM range (% of total)

Number of genes

 

Linseed oil treatment

Safflower oil treatment

Very highly expressed

>2 %

7

7

Highly expressed

0.1 to 1.99 %

15

17

Medium expressed

0.01 to 0.099 %

219

212

Lowly expressed

0.001 to 0.0099 %

2574

2522

bVery lowly expressed, retained

<0.0099 %

8508

8393

cVery lowly expressed, not used in DE

<0.0099 %

7369

7314

  1. aReads per kilo base per million mapped reads (RPKM)
  2. b,cVery lowly expressed genes had RPKM values of <1 %. Those that had read counts >1CPM (counts per million) in at least 10 libraries were used in differential gene expression (DE) analysis while those with >1 CPM in less than 10 libraries were not used