BMC Genomics

Table 6 Results of qPCR validation of RNA-sequencing data

From: Transcriptome adaptation of the bovine mammary gland to diets rich in unsaturated fatty acids shows greater impact of linseed oil over safflower oil on gene expression and metabolic pathways

Gene	qPCR				RNA-seq
Gene	Fold change D-14 vs D7	Fold change D7 vs D28	Fold change D-14 vs D28	Overall P-value	Fold change D-14 vs D28	P-value	FDR P-value
Linseed oil treatment
CSN2	1.011	−1.410	−1.397	0.3644	−1.003	0.9856	0.9956
ACE2	1.021	1.915**	1.955	0.0450	2.006**	1.85E-05	0.0050
FASN	−1.156	−1.167	−1.348	0.5653	−1.520*	0.0010	0.0740
FBP2	3.921**	3.248**	12.735**	0.0012	11.023**	1.95E-09	4.33E-06
MROH2B	−1.957	−1.506	−2.950	0.4434	−4.154**	0.0002	0.0229
RASD1	−1.376	−1.284	−1.767	0.2045	−1.719**	0.0004	0.0472
SREBF1	−1.934*	−1.080	−2.088**	0.0297	−1.645**	7.36E-07	0.0005
TC2N	1.124	1.998*	2.246**	0.0358	2.272**	1.82E-06	0.0009
TIEG2	1.069	2.382**	2.546**	0.0070	2.591**	8.01E-12	4.45E-08
UCP2	1.065	2.807**	2.989**	0.0019	2.256**	0.0002	0.0228
Safflower oil treatment
CSN2	−1.233	−1.048	−1.292	0.5687	−1.003	0.9856	0.9956
ACE2	1.830*	1.551	2.838**	0.0241	3.061**	1.28E-07	0.0008
FASN	−1.420**	−1.318**	−1.873**	0.0020	−1.625*	0.0006	0.0854
FBP2	3.583*	3.980*	14.261**	0.0059	6.302**	2.52E-05	0.0111
MROH2B	−1.634	−1.972	−3.215*	0.1064	−2.784**	1.06E-05	0.0083
RASD1	1.162	−1.495	−1.287	0.1605	−1.878**	1.71E-06	0.0020
SREBF1	−1.859	−1.420	−2.646**	0.0123	−1.937**	4.62E-06	0.0042
TC2N	1.219	1.831	2.232*	0.0584	1.852*	0.0007	0.0946
TIEG2	1.187	1.547	1.837*	0.0903	2.114**	1.49E-05	0.0097
UCP2	1.002	1.948**	1.952	0.0475	2.362**	1.91E-06	0.0021

*FDR/P-value < 0.1 **FDR/P-value < 0.05

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