Fig. 2

Point mutations in the Pr77 sequence in stable transfectants for analysis of the involvement of these nucleotide positions in the transcription capacity of the Pr77 promoter. The DPE motif is shadowed in the Pr77 L1Tc sequence. Nucleotides are numbered from the L1Tc transcription start site (+1). Conserved nucleotides among L1Tc and NARTc from T. cruzi, and ingi and RIME from T. brucei, are indicated by asterisks below the Pr77 sequence. Mutant names (from M1 to M17) are shown on the left hand side and the corresponding nucleotide substitution and position indicated for each mutant. Also on the left hand side it is indicated whether the DNA secondary structure is not affected (NA), slightly affected (+) or very affected (+++) by the point mutation, as predicted by the Mfold program