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Fig. 3 | BMC Genomics

Fig. 3

From: The Trypanosomatid Pr77-hallmark contains a downstream core promoter element essential for transcription activity of the Trypanosoma cruzi L1Tc retrotransposon

Fig. 3

Analysis of transcriptional activity of Pr77-derived sequences in T. cruzi stable transfectants by northern blotting. a, b, c, d Cytoplasmic RNA from T. cruzi stable transfectants eletroporated with pTEXPr77Luc or pTEXPr77Mut1-17Luc, was electrophoresed in 1 % denaturing agarose gel, transferred to nylon membranes, and hybridized with 32P-labelled Luc (luc) and KMP11 (kmp11) coding sequences as probes. The ethidium bromide staining of ribosomal RNAs is shown below each panel (a-d). Cytoplasmic RNA from wild type Y epimastigotes and parasites transfected with the pTEXLuc construct were used as negative and positive controls (C- and C+ respectively). The northern blots were performed three times. The percentage of transcribed Luc mRNA when using the pTEXPr77Luc construct is shown below the luc hybridization panel as TR (%)

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