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Fig. 5 | BMC Genomics

Fig. 5

From: The Trypanosomatid Pr77-hallmark contains a downstream core promoter element essential for transcription activity of the Trypanosoma cruzi L1Tc retrotransposon

Fig. 5

Determination of the in vivo transcription start site of Luc mRNA by primer extension. Total RNAs from T. cruzi transfected with the pTEXPr77Luc (Pr77), pTEXPr77M5Luc, pTEXPr77M7Luc or pTEXPr77M15Luc constructs (M5, M7 and M15) were used for primer extension of LUC mRNAs employing a γ-ATP32 Luc antisense primer (LUC28rev). The cDNA products were resolved in 8 M urea 8 % polyacrylamide gels. The full length extension products (117 nt in length), consistent with the TSS (FL) of Pr77 (nt +1) and 18-mer radiolabelled oligo LUC28rev, are indicated

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BMC Genomics

ISSN: 1471-2164

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