Fig. 1
S. aureus Tager 104 Bacteremia Leading to Multi-organ Septic Foci. a Gram staining of C57BL/6 kidney 8 days after injection of 5 × 108 CFU S. aureus Tager 104. b Higher magnification (400× total) of the indicated by the box in panel a. Arrows indicate the protective fibrin layer that allows the bacteria to thrive in the glomerulus (GM) without the threat of clearance by phagocytic immune cells (see area immediately surrounding infected GM shown in Panel a). c Adjacent section of the same kidney stained with Hematoxylin and Eosin (H&E); please note the GM is at a slightly earlier stage of the disease. Kidney architecture including the collecting ducts (CD), the distal tubule (DT), and blood cells (red). d Gram staining shows bacteria (purple) in all organs examined; splenic abscesses also show thickening of the marginal zone and increased fibrin deposition (arrows). e Adjacent section stained for H&E showing the splenic architecture including the white pulp (WP) region, marginal zone (MZ) and red pulp (RP) region