Fig. 2

Microdissection of different regions from chicken lampbrush chromosomes (examples). Microdissection of single chromomeres from LBC1 (a, a’), whole chromosome W from the sex bivalent (b, b’), the structure «Spaghetti marker» (SM) from LBC2 (c-c”) and the special loop «Lumpy loop» (LL) from LBC3 (d-d”). (b, c, d) – phase contrast images of corresponding lampbrush chromosomes before microdissection procedure. (a, b’, c’, d’) – phase contrast images of corresponding lampbrush chromosomes after microdissection procedure. Black arrows point to the regions subjected to microdissection. (a’, c”, d”) – images of corresponding lampbrush chromosomes are counterstained with DAPI. (a”, b”, c”’, d”’) – Cytological maps of chromomere-loop patterns [53] showing dissected regions for LBC1, ZW bivalent, LBC2, LBC3, respectively. (e-e”) Transfer of microdissected material with a glass-needle into a Pasteur pipette containing a collection drop. Scale bar = 10 μ. PBL11, TGL, TBL-marker loops