Rapid identification of causative insertions underlying Medicago truncatula Tnt1 mutants defective in symbiotic nitrogen fixation from a forward genetic screen by whole genome sequencing

Table 3 Summary of whole genome sequencing data and coverage estimates

	Tnt1 mutant line
	NF11217	NF10547
Total number of raw reads (millions)	196.31	184.11
Total nucleotides of raw reads (Giga bases)	17.67	16.57
Total number of clean reads (millions)^a	188.03	176.77
Total nucleotides of clean reads (Giga bases)	16.92	15.91
Total genome coverage of clean reads^b	44X	41X

^aAfter removing the adapter sequences and reads that contain >50 % low quality bases
^bTotal genome coverage was calculated based on the estimated Medicago truncatula A17 genome size of 384 Mb Krishnakumar et al., [26] using the formula clean bases/reference genome size

ISSN: 1471-2164