Fig. 8

Changes in cell wall cellulose content in various cell types upon cold treatment. Plants of three maize inbred lines were grown at 24 °C/22 °C (control, day 0) and transferred to 14 °C/12 °C for seven days. Third leaves were collected at time points indicated and cross-sections were stained for cellulose with Calcofluor White. Fluorescence was quantitated under a confocal microscope and expressed as normalized corrected total fluorescence (NCTF) as described in Materials and Methods. All values are expressed relative to the mean fluorescence at day 0 for a given line and cell type set at 1. a Vascular tissue; b. Bundle sheath; c. Kranz mesophyll; d. Whole veins. Data are means ± SD for at least 10 veins from three plants from three independent experiments