Fig. 1

RT-qPCR experiments for a selected set of lncRNAs in brains. a 22 novel lncRNAs discovered in the present study were selected for validation. RpL32 (a coding gene) and roX1 (a non-coding gene) were included as positive controls. The horizontal line indicated − delta Ct ≥ 1. The rectangle indicated the five lncRNAs with considerably low expression, and was tested again by the second RT-qPCR experiment shown in (b). b The five lncRNAs from the rectangle of (a) were tested again by RT-qPCR with twofold amount of template cDNA. Ten FlyBase lncRNAs were included for comparison. The three FlyBase lncRNAs highlighted by the orange stars were selected because their RPKM values in our brain RNA-seq data was 0