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Fig. 6 | BMC Genomics

Fig. 6

From: Integrating RNA-seq and ChIP-seq data to characterize long non-coding RNAs in Drosophila melanogaster

Fig. 6

Procedures for discovering novel lncRNAs from RNA-seq data of the present study. The sequencing read datasets of poly(A)-enriched RNA and total RNA were respectively mapped to the reference genome sequence using TopHat and Cufflinks. Putative lncRNAs were then discovered by Cuffcompare, followed by coding potential estimation and rRNA exclusion. Sequencing reads were again mapped to the set of putative lncRNAs to construct the final set of novel lncRNAs

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