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Fig. 2 | BMC Genomics

Fig. 2

From: Highly diverse nirK genes comprise two major clades that harbour ammonium-producing denitrifiers

Fig. 2

Unrooted bifurcating neighbour joining tree of full-length NirK sequences obtained from whole genomes. NirK Clade I encompassed Alphaproteobacteria and some Gamma- and Betaproteobacteria, while NirK Clade II comprised Firmicutes, Actinobacteria, Euryarchaea, Chloroflexi, Bacteroidetes, Nitrospirae, Gemmatimonadetes, Planctomyces, Verrucomicrobia and Proteobacteria. The same bifurcating tree in cladogram format with an estimation of reliability based on bootstrap analysis can be found in supplementary information (Additional file 1). Physiologically characterized denitrifying strains are indicated by â–¼: Bacillus azotoformans LMG 9581, Bacillus bataviensis LMG 21833 [39], Haloferax denitrificans ATCC 35960 [40], Hyphomicrobium denitrificans ATCC 51888 [77], Shewanella loihica PV-4 [44], Shewanella denitrificans OS217 [78], Rhodobacter sphaeroides ATCC 17025 [79], Alcaligenes faecalis S-6 [80]. The grey ovals represent the two previously proposed clades of NirK sequences

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