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Table 1 qRT-PCR retinal gene expression results in xlpra2, rcd1, and erd mutants compared to normal retinas

From: Photoreceptor proliferation and dysregulation of cell cycle genes in early onset inherited retinal degenerations

  Ages
Gene 3 wks 5 wks 7 wks 16 wks
Cell cycle: cyclins   
CCNA1 n.s n.s n.s +4.4x in rcd1
+2.2x in xlpra2
+3.0x in erd (11.9–14.1 w)
CCNA2 n.s n.s +2.0x in rcd1 n.s
+2.2x in xlpra2
+2.2x in erd (6.4 w)
+3.2x in erd (8.3–9.9 w)
CCNB1 n.s n.s n.s +3.3x in rcd1
+2.8x in xlpra2
CCND1 n.s n.s n.s +2.4x in rcd1
+2.7x in xlpra2
CCND3 n.s n.s n.s +2.8x in rcd1
CCNE1 n.s +2.4x in rcd1 +2.7x in rcd1 +5.5x in rcd1
+4.1x in xlpra2 +5.1x in xlpra2
+2.8x in erd (6.4 w) +10x in erd (11.9–14.1 w)
+5.7x in erd (8.3–9.9 w)
Cell cycle: cyclin-dependent kinases   
CDK1 n.s −2.1x in rcd1 −13.3x in rcd1 −2.2x in rcd1
−10.2x in xlpra2 −3.4x in xlpra2
−8.4x in erd (6.4 w) −6.0x in erd (11.9–14.1 w)
−6.9x in erd (8.3–9.9 w)
CDK2 +2.7x in rcd1 n.s n.s +3.4x in rcd1
CDK4 +2.2x in rcd1 n.s n.s +2.4x in rcd1
CDK6 +3.6x in rcd1 n.s n.s +2.9x in rcd1
+2.5x in xlpra2 +2.1x in xlpra2
Cell division cycle phosphatases   
CDC25A n.s n.s n.s n.s
CDC25B n.s n.s n.s n.s
CDC25C n.s n.s n.s +2.1x in rcd1
+2.7x in xlpra2
Cyclin-dependent kinase inhibitors   
CDKN1A n.s n.s n.s n.s
CDKN1B n.s n.s n.s n.s
CDKN2A −3.8x in rcd1 n.s +3.0x in erd (8.3–9.9 w) n.s
Cell cycle transcription regulators   
BMI1 n.s n.s n.s n.s
E2F1 +2.0x in rcd1 n.s n.s +6.2x in rcd1
+2.6x in xlpra2
+3.7x in xlpra2
RB1 +2.1x in rcd1 n.s n.s n.s
+5.5x in xlpra2
Hippo signaling/NDR kinases   
LATS1 n.s n.s n.s +2.6x in erd (11.9–14.1 w)
LATS2 n.s n.s n.s n.s
MOB1A n.s n.s n.s +2.5x in erd (11.9–14.1 w)
NDR1 n.s n.s n.s +2.1x in erd (11.9–14.1 w)
STK38L (NDR2) (exons 4–5) n.s n.s No expression in erd No expression in erd
STK38L (NDR2) (exons 6–7) n.s n.s n.s n.s
Eye development regulation via proliferation/apoptosis  
PAX6 n.s n.s −2.5x in erd (6.4 w) +2.2x in rcd1
PCNA n.s n.s n.s n.s
PR function    
GRK1 n.s −2.0x in rcd1 −3.9x in rcd1 −3.3x in rcd1
−2.3x in xlpra2
RBP3 n.s n.s n.s n.s
RCVRN n.s n.s −2.6x in rcd1 −2.4x in rcd1
−2.2x in xlpra2
−2.0x in erd (11.9–14.1 w)
PR development/structure    
CRB1 n.s n.s n.s n.s
NRL n.s −2.3x in rcd1 −2.0x in rcd1 −3.0x in rcd1
−3.2x in erd (6.4 w) −2.0x in xlpra2
−3.7x in erd (8.3-9.9 w) −5.8x in erd (11.9–14.1 w)
NR2E3 n.s −2.0x in rcd1 −2.8x in erd (6.4 w) n.s
RDS n.s −2.5x in rcd1 −2.0x in rcd1 −3.0x in rcd1
  1. Statistically significant (p < 0.05 and FC > +/−2) differences in retinal gene expression are reported in comparison to normals at different ages (rcd1 and xlpra2: 3, 5, 7, 16 wks; erd: 6.4, 8.3–9.9, 11.9–14.1 wks). The examined genes are reported in alphabetical order within functional groups. The complete list of genes tested is available as Additional file 5. n.s = not statistically significant; + = up-regulated and - = down-regulated compared to normal